Researcher of the laboratory of Biochemistry, Hilda Alfaro, has your learning experience in the technique of nanodisco in the University of Gronigen, Netherlands

Researcher of the laboratory of Biochemistry, Hilda Alfaro, has your learning experience in the technique of nanodisco in the University of Gronigen, Netherlands

At the end of 2017, the researcher of the laboratory of Biochemistry, Hilda Alfaro-Valdes traveled to the Dutch University of Groningen to work in experimental research with Professor Dr. Arnold Driessen, Professor of molecular microbiology that University. On the occasion, was to learn the technique of nanodisco, as an alternative to follow with the experiments of optical tweezers that Professor Christian Wilson has in his laboratory.

As she tells "optical tweezers, is an instrument used to measure forces." In our laboratory are used to determine the forces that could be generating a protein called BiP, which is involved in the process of post-translational translocation of proteins". Note that "detergent that is used to keep the channel through which passes the protein (complex sec61) generates micelles, which disrupts the measurement." The nanodiscos would be then an alternative to maintain the complex stable sec61 in the nanodisco without the use of detergent and thus continue with experiments in optical tweezers".

Professor Driessen is not unknown to our faculty. He has been visiting professor in the Department of Biochemistry and Molecular Biology at several occasions, being his last visit in September last year 2017, occasion in which gave the lecture "Dynamics of membrane localization of the protein motor translocation "Dry in e. coli", among other activities. On that occasion, corresponded to Hilda present to Professor Driessen.

Hilda Alfaro Valdés, 31 years old and a native of Iquique, engineer in biotechnology from the Universidad Arturo Prat, arrived at work a while ago with Professor Christian Wilson for help in the laboratory on the technical side, given their knowledge in molecular techniques. Because she herself said, he always liked the research, Professor Wilson integrated to one of the projects, which left her in charge and for which it is developing her Magister thesis in biochemistry, which is studying in our school. This aims to be able to determine the mechanism mecanoquimico beep that is involved in the pursuit of protein production.

Hilda had previously made their practice of engineer in biotechnology with the Professor Alfonso Paredes and then did his thesis that career in the Faculty of medicine in cardiac hypertrophy. It was in that instance that she learned about molecular techniques.

His experience in the leading Dutch University emphasizes that all learning was in English. "All the interns came from different countries, Dutch English was difficult to understand but I managed to make me understand and I met a Mexican girl who helped me." It adds that in English also presented their results and was able to show our country and its places, especially Iquique, his hometown. "They were very impressed because many did not know Chile," he said.

Some of the aspects that the researcher highlighted the University culture of the University of Groningen is the full-time. "There if you are in the PhD you do just that, or only you investigate, you're hired for that." Here we do everything", explained. Similarly, stressed that "are super efficient since they work 9 to 5".

In relation to the way of life, called him the attention that all went bicycling as well as that larger houses but rather all would not be equal. "That's why it is easy to get lost", said Hilda. "I liked it enough to be an orderly society, people are very respectful," she said excited.

Office of Extension and communication. 15 March of 2018.