Proteins & Proteomics Protocols From Cold Spring Harbor

Proteins & Proteomics Protocols From Cold Spring Harbor

Free — Read these key Proteins and Proteomics protocols:

Immunometric Antibody Sandwich Enzyme-Linked Immunosorbent Assay
Thomas O. Kohl and Carl A. Ascoli
The double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) is preferentially used to determine the concentration of unknown antibody in a sample. Pure antigen is not required in this assay; however, the use of a reporter-labeled detection antibody is essential.

Proteomic Analysis of Protein Posttranslational Modifications by Mass Spectrometry
Danielle L. Swaney and Judit Villén
This protocol describes several of the most influential PTMs and discussed are the fundamentals of proteomics experiments and PTM purification methods.

Elementary Protein Analysis inSchizosaccharomyces pombe
Agnes Grallert and Iain M. Hagan
In this protocol issues encountered during the analysis of fission yeast protein preparations are discussed.

Prion Transfection of Yeast
Herman K. Edskes, Dmitry Kryndushkin, Frank Shewmaker, and Reed B. Wickner
The double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) is preferentially used to determine the concentration of unknown antibody in a sample. Pure antigen is not required in this assay; however, the use of a reporter-labeled detection antibody is essential.

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