Live webinar: Optimize whole genome sequencing for challenging samples
•Title: New technologies for low-input whole genome sequencing
In this webinar, we will present methods to optimize library construction to efficiently convert small amounts of DNA samples into sequencing libraries, especially for whole genome sequencing applications. We will highlight enzymatic DNA fragmentation technology, ultralow-input ligation chemistry and Phi29-based MDA technology for whole genome amplification. Join us and learn how you can apply these technologies to your NGS workflow!
How fast is your amplicon library prep?
With targeted – or amplicon – sequencing, researchers can zero-in on specific regions to find rare mutations or for 16s rRNA microbial profiling. But library prep can take 2–3 hours to complete. Can we decrease this time to just 30 minutes?
Watch: All-enzymatic library prep
Fragmentation is the first step to generating an NGS library, so what are your options? Learn the difference between mechanical and enzymatic fragmentation, and how QIAseq FX technology works.
From single cells to CNV insights
Recently, a study used multiple displacement amplification (MDA) for CNV detection with whole genome and low-pass sequencing, overcoming challenges with single-cell sequencing that are often caused by traditional PCR amplification.
Sequencing the microbiome
After extracting nucleic acids from microbial samples, what is the best way to analyze the microbiome? We discuss 2 types of sequencing – amplicon and whole genome – to analyze samples and discover novel insights.