This course gives students a hands-on experience synthesizing and using protein-small molecule conjugates to visualize extracellular proton accumulation and depletion. This course is based on Bill Kobertz laboratory’s newest and unpublished reagent (WGA-pHRho); thus, it has not been taught before. The lab will be ~ 3 hrs a day, depending on the successes and/or failures of the experiments.
University of Massachusetts Medical School
Date: October 8-19, 2018
Fundamentals of Fluorescence – Chemical Synthesis of Oligonucleotides – Unnatural Amino Acids – Chemical Genetics – Post-translational Modifications of Proteins