COURSE INTRODUCTION TO THERMODYNAMIC ANALYSIS ADVANCED INTERACTION BETWEEN BIOMOLECULES

COURSE INTRODUCTION TO THERMODYNAMIC ANALYSIS ADVANCED INTERACTION BETWEEN BIOMOLECULES

Duration: 23 to January 25, 2017
Load time:

  • Theoretical: 17 pm
  • Seminar: 3 hours

Dictation: Dr. Sergio B. Kaufman, University of Buenos Aires, Argentina
Conditions of admission: professionals and graduates in Biochemistry and related areas. Graduate students from the area.
Room: Room 07 lecture, Faculty of chemical and pharmaceutical sciences, University of Chile. Santos Dumont 964, Independencia, Santiago, Chile
Hours: 9-12 h and from 14 to 17.30 hrs
Cost: free
Registration: Until 30 December 2016 by sending email to yitowilson@gmail.com CV and explaining interest in the course.
Quota: 35 students

OBJECTIVES

Provide a comprehensive conceptualization of the application of theoretical tools and experimental design for the characterization of joining process of ligands to macromolecules of biological interest.

ANALYTICAL PROGRAM:

1. interactions between ligands and biomolecules in balance. Practical characterization of the balance. Thermodynamic variables needed for the description of the processes of interaction. Experimental methods for obtaining of isotherms of Association (binding curves).

2. microscopic models of independent association. Macroscopic and microscopic constants. Union of ligands identical and independent sites. Occupation of the same sites by different ligands. Competition between two ligands to the same place with equal affinity. Different sites for the same ligand. Thermodynamic models of union restrictions.

3. interactions between sites. Cooperativity. free energy and interaction energy of coupling (coupling energy). Non-specific binding of macroligandos to biomolecules: protein - nucleic acid interactions.

4. kinetics of the union of ligands to biomolecules in pre-equilibrio conditions. Conditions of pseudo-orden. Kinetic models of union related to conformational changes: induced fit and conformational selection.

Seminar: Isotherms of union obtaining model independent from spectroscopic titration curves.

Bibliography

(a) textbooks

Tanford C. Physical Chemistry of Macromolecules. Cap 8 Wiley, New York 1961 Cantor CR, Schi.PR Biophysical Chemistry, Cap 15 and 16, Freeman , New York, 1980 Weber G Protein Interactions, Chapman, New York 1992 Wyman J, Gill SJ Binding and Linkage. Functional Chemistry of Biological Macromolecules. University Science Books, Mill Valey, CA, 1990. Hill TL Cooperativity Theory in Biochemistry, Springer, New York, 1985 Ladbury JE, Doyle ML Biocalorimetry 2, Wiley, New York, 2004.

b) Artículos de revisión

Bujalowski W. Thermodynamic and Kinetic Methods of Analyses of Protein-Nucleic Acid Interactions. From Simpler to More Complex Systems. Chem Rev 106:556-606, 2006 Ciu Q, Karplus M. Allostery and cooperativity revisited. Prot Sci 17:1295-1307, 2008

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